1. Product introduction Trypsin is a serine protease that selectively hydrolyzes peptide chains in proteins consisting of the carboxyl terminations of lysine or arginine. This product is isolated and purified from recombinant Pichia pichia and then freeze-dried to obtain recombinant trypsin powder. This product does not contain enzymes such as DFP, PMSF and TLCK, and has the same activity and specificity as trypsin. This product is also active by serine protease preparations such as PMSF and TLCK, metal ion chelating agents such as EDTA, etc. We offer recombinant trypsin (cell culture grade catalog number: P313) and recombinant trypsin (pharmacopeial grade catalog number: P303). 2. Product advantages High purity: higher than live; Host protein residues are less than the biologics limit. Complies with the standards of the Chinese Pharmacopoeia 2020 edition and USP43. Compliance: Production facilities and production environments comply with relevant regulatory requirements and GMP guidelines. Complete quality documentation: Regulatory support documents are available upon request. 3. Product characteristics Properties: white or off-white freeze-dried powder Molecular weight: 23.5KD±2.4KD Purity: ≥95% (SDS-PAGE) Enzyme specific activity: ≥3800USP unit/mg pro. HPLC purity: α-trypsin shall not be greater than 20%; β-trypsin must not be less than 70% Foreign-based DNA residue: ≤100ng/mg pro. Bacterial protein residue: ≤100ng/mg pro. Optimal pH: 7.0-9.0 Solubility: soluble in water or buffer 4. Product use (recombinant trypsin, cell culture grade) Cell culture: Seedling production, cell culture Digestion of cells cultured by microcarrier method c. Dry cells are gentle digestion, etc In terms of recombinant proteins: a. Insulin production Protein analysis 5. Precautions and Disclaimer a. This product has moisture absorption and should be taken after room temperature equilibrium. b. This product shall not be used for clinical diagnosis and treatment.