1. Product Introduction The product is a recombinant lysyl endonuclease powder which is isolated and purified from recombinant E. coli and then freeze-dried.This product is a biochemical grade genetically engineered enzyme. It does not contain enzyme inhibitors such as DFP, PMSF and TLCK. It has the same activity and specificity as natural lysyl endo-enzyme and can be used in biological products such as insulin and GLP-1. 2. Product Features. Appearance: White or off-white powder MW: 36kD±3.6kD Purity: Single main band (SDS-PAGE) with a molecular weight of 27.7 kD ± 2.8 kD Protein content: 30%-70% Residue DNA: ≤100ng/mg pro. Residue HCP: ≤100ng/mg pro. Optimum pH: 8.5~9.5. Solubility: Soluble in the buffer. (Enzyme activity definition: At 30 ° C, pH 9.5, the amount of enzyme that produces 1 μmol of p-nitroaniline per minute for the catalytic substrate is 1 AU.) 3. Product Usage a. Production of biological products, such as insulin and analog production, production of GLP-1 analogues. b.Protein structure and function studies, such as protein mass spectrometry, sequencing, peptide mapping analysis. c.Production of small molecular proteins or peptides, such as cosmetic peptides, antimicrobial peptides, food flavor peptides, and the like. d. Enzymatic synthesis of Lys-X compounds. e.The cultivation of stem cells and primary cells 4. Transport and Storage Stability Transport stability: It is transported in an ice pack to keep it active. Storage stability: Store at -15~-20°C, stable within 24 months; Store in -15 with a solution of enzyme concentration greater than 1mg/ml in Tris-HCl buffer pH 8.0 to pH 11.0~-20 ° C, repeated freeze-thaw 10 times, no loss of activity. It is recommended not to exceed 7 days after storage at 2~8°C, and no more than 30 days for -15~-20°C. 5. Notice and Disclaimer This product may self-digest when the temperature exceeds 37 °C.Most of the viability is retained in 2M urea or 0.1% SDS. This product has moisture absorption and should be taken after room temperature balance. This product is for research use only and should not be used for diagnosis or treatment of animals or humans.